IMAGE cDNA clones, UniGene clustering, and ACeDB: an integrated resource for expressed sequence information.

NEXTDB: The Expression Pattern Map Database for C.elegans

We have developed a WWW-based database, named NEXTDB, to integrate all the information of ESTs (tag-sequences of cDNA clones) and gene expression patterns of C.elegans which are being produced and analyzed in this laboratory. NEXTDB incorporates and processes raw data of tag sequencing and classi es them into unique cDNA groups by comparing the 3'-tags. The database contains the information on ...

A Database System for cDNA Expression Profile Using ESTs of Oligo-Capping Clones and UniGene

library construction induced mutation rate from expressed sequence tag collections

Estimation of population heterozygosity and library construction induced mutation rate from expressed sequence tag collections. Abstract: Unigene alignments obtained from cDNA libraries made using multiple individuals are not currently used to estimate population heterozygosity, as they are known to harbor mutations created during library construction. We describe an estimator of population het...

The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system.

The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs we...

The Gene Resource Locator : Integrated Annotation of the Draft Human Genome

According to recent several reports [1, 5], it seems clear that the number of human genes is only several times those of model species such as C.elegans. This indicates that correlations between species complexity and gene number are not stringent and that other factors determine complexity, such as alternative splicing, tissue-specific expression, cis-regulatory mechanisms and other global pro...